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Product last modified at: 2025-04-03T11:45:48.776Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Phospho-Met (Tyr1234/1235) Antibody #3126

Filter:
  • WB
  • IP
Western Blotting Image 1: Phospho-Met (Tyr1234/1235) Antibody
Western blot analysis of extracts from mIMCD-3, A431 and Vero cells, untreated or HGF-stimulated (40 ng/ml for 5 minutes), using Phospho-Met (Tyr1234/1235) Antibody.

To Purchase # 3126

Cat. # Size Qty. Price
3126S 100 µl
$357

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 145
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Phospho-Met (Tyr1234/1235) Antibody detects endogenous levels of Met when dually phosphorylated at tyrosine 1234/1235, and when singly phosphorylated at either Tyr1234 or Tyr1235. This antibody cross-reacts with activated Ron, EGF, PDGF, insulin and FGF receptors.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1234/1235 of human Met. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Met, a high affinity tyrosine kinase receptor for hepatocyte growth factor (HGF, also known as scatter factor) is a disulfide-linked heterodimer made of 45 kDa α- and 145 kDa β-subunits (1,2). The α-subunit and the amino-terminal region of the β-subunit form the extracellular domain. The remainder of the β-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl, and PI3 kinase (3). These fundamental events are important for all of the biological functions involving Met kinase activity. The addition of a phosphate at cytoplasmic Tyr1003 is essential for Met protein ubiquitination and degradation (4). Phosphorylation at Tyr1234/1235 in the Met kinase domain is critical for kinase activation. Phosphorylation at Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (5). Research studies have shown that altered Met levels and/or tyrosine kinase activities are found in several types of tumors, including renal, colon, and breast. Thus, investigators have concluded that Met is an attractive potential cancer therapeutic and diagnostic target (6,7).

Pathways

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For Research Use Only. Not For Use In Diagnostic Procedures.
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