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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Phospho-MSK1 (Ser360) Antibody #9594

Filter:
  • WB
Western Blotting Image 1: Phospho-MSK1 (Ser360) Antibody
Western blot analysis of extracts from 293 cells transfected with MSK1 or various mutants of MSK1, then treated with TPA (200 nM) and analyzed using Phospho-MSK1 (Ser360) Antibody.

To Purchase # 9594

Cat. # Size Qty. Price
9594S 100 µl
$357

Supporting Data

REACTIVITY H
SENSITIVITY Transfected Only
MW (kDa) 90
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Phospho-MSK1 (Ser360) Antibody detects transfected MSK1 only when phosphorylated at Ser360. This antibody does not recognize MSK1 phosphorylated at other sites, nor does it cross-react with phosphorylated RSK1, RSK2 or RSK3.

Species Reactivity:

Human

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser360 of human MSK1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

MSK1, a mitogen and stress activated protein kinase, is activated by Erk as well as p38 MAPK in response to growth factors and cellular stress, respectively (1). MSK1 resembles RSK because it has two kinase domains connected by a regulatory linker region (2). Phosphorylation of RSK1 at Ser364 and Ser381 is critical for RSK1 activity (3). These sites are analogous to Ser360 and Ser376 of MSK1, which may be important for MSK1 activity as well.

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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