Phospho-NEDD4L (Ser342) (D16D6) Rabbit mAb #12146
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 110,135 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
NEDD4L can be phosphorylated at Ser342 and Ser448 by several AGC kinase family members including SGK1, Akt, and PKA (8-11). Phosphorylation at these sites inhibits NEDD4L function as a suppressor of ENaCs and as a regulator of TGF-β signaling through its effects on Smad2/3 binding (8-11).
- Kumar, S. et al. (1992) Biochem Biophys Res Commun 185, 1155-61.
- Harvey, K.F. and Kumar, S. (1999) Trends Cell Biol 9, 166-9.
- Dinudom, A. et al. (1998) Proc Natl Acad Sci USA 95, 7169-73.
- Goulet, C.C. et al. (1998) J Biol Chem 273, 30012-7.
- Staub, O. et al. (1996) EMBO J 15, 2371-80.
- Kuratomi, G. et al. (2005) Biochem J 386, 461-70.
- Harvey, K.F. et al. (1998) J Biol Chem 273, 13524-30.
- Gao, S. et al. (2009) Mol Cell 36, 457-68.
- Lee, I.H. et al. (2007) J Biol Chem 282, 29866-73.
- Snyder, P.M. et al. (2004) J Biol Chem 279, 45753-8.
- Debonneville, C. et al. (2001) EMBO J 20, 7052-9.
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