Phospho-NMDA Receptor 2A (GluN2A) (Tyr1246) Antibody #4206
- WB
Supporting Data
| REACTIVITY | M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 180 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
EphrinB2 binding to the receptor EphB leads to the activation of Src family tyrosine kinases, which phosphorylate NMDAR2B at Tyr1252, Tyr1336, and Tyr1472. In turn, phosphorylated NMDAR2B enhances the ability of the functional NMDA receptor to regulate Ca2+ influx in response to glutamate (7). The phosphorylation site of NMDAR2A at Tyr1246 is the conserved site of NMDAR2B at Tyr1252 and was independently identified at Cell Signaling Technology (CST) using PhosphoScan®, a CST MS/MS platform for phosphorylation site discovery. Phosphorylation of NMDAR2A at Tyr1246 was observed in extracts isolated from ischemic rat brain. Please visit PhosphoSitePlus®, a CST modification site knowledgebase, at phosphosite.org for additional information.
- Liu, X.B. et al. (2004) J Neurosci 24, 8885-95.
- Westphal, R.S. et al. (1999) Science 285, 93-6.
- Tingley, W.G. et al. (1997) J Biol Chem 272, 5157-66.
- Hisatsune, C. et al. (1997) J Biol Chem 272, 20805-10.
- Raman, I.M. et al. (1996) Neuron 16, 415-21.
- Makhinson, M. et al. (1999) J Neurosci 19, 2500-10.
- Takasu, M.A. et al. (2002) Science 295, 491-495.
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