Phospho-Optineurin (Ser177) (E8L9I) Rabbit mAb #31304
- WB
- IP
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 75 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Phosphorylation of optineurin at serine 177 by TBK1 enhances binding to LC3 and promotes autophagic clearance (8). Additional studies also suggest that serine 177 is phosphorylated during mitosis by PLK1 (9). In addition to serine 177, TBK1 also phosphorylates optineurin at serine 473 and 513, which can enhance its binding to ubiquitin chains and promote mitophagy (5,6).
- Ying, H. and Yue, B.Y. (2016) Exp Eye Res 144, 73-80.
- Slowicka, K. et al. (2016) Trends Immunol 37, 621-33.
- Markovinovic, A. et al. (2017) Prog Neurobiol 154, 1-20.
- Moore, A.S. and Holzbaur, E.L. (2016) Proc Natl Acad Sci U S A 113, E3349-58.
- Richter, B. et al. (2016) Proc Natl Acad Sci U S A 113, 4039-44.
- Heo, J.M. et al. (2015) Mol Cell 60, 7-20.
- Liu, Z. et al. (2014) Cancer Cell 26, 106-20.
- Wild, P. et al. (2011) Science 333, 228-33.
- Kachaner, D. et al. (2012) Mol Cell 45, 553-66.
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