Phospho-p38 MAPK Pathway Antibody Sampler Kit #9913
Product Information
Kit Usage Information
Protocols
- 3007: Western Blotting
- 4511: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Paraffin), Immunofluorescence, Flow
- 7074: Western Blotting
- 9595: Western Blotting, Immunoprecipitation (Agarose)
- 9709: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence, Flow
- 12280: Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence
- 15411: Western Blotting, Flow
Product Description
Specificity / Sensitivity
Source / Purification
Background
Four residues (Thr25, Thr222, Ser272 and Thr334) of MAPKAPK-2 are phosphorylated by p38 in an in vitro kinase assay (3). Phosphorylation at Thr222, Ser272 and Thr334 seems to be essential for the activity of MAPKAPK-2 (3). Activated MAPKAPK-2 can in return phosphorylate HSP27 at serines 15, 78 and 82 (3,9). Phosphorylation of HSP27 causes a change in the tertiary structure of HSP27, which shifts from large homotypic multimers to dimmers and monomers (10). It has been illustrated that phosphorylation and increased concentration of HSP27 modulate actin polymerization and reorganization (11,12).
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- Han, J. et al. (1994) Science 265, 808-11.
- Lee, J.C. et al. (1994) Nature 372, 739-46.
- Freshney, N.W. et al. (1994) Cell 78, 1039-49.
- Raingeaud, J. et al. (1995) J Biol Chem 270, 7420-6.
- Zervos, A.S. et al. (1995) Proc Natl Acad Sci U S A 92, 10531-4.
- Zhao, M. et al. (1999) Mol Cell Biol 19, 21-30.
- Yang, S.H. et al. (1999) Mol Cell Biol 19, 4028-38.
- Cuenda, A. et al. (1995) FEBS Lett 364, 229-33.
- Kumar, S. et al. (1999) Biochem Biophys Res Commun 263, 825-31.
- Landry, J. et al. (1992) J Biol Chem 267, 794-803.
- Rogalla, T. et al. (1999) J Biol Chem 274, 18947-56.
- Lavoie, J.N. et al. (1993) J Biol Chem 268, 24210-4.
- Rousseau, S. et al. (1997) Oncogene 15, 2169-77.
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