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Render Timestamp: 2024-11-22T12:04:28.967Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-08-01 15:26:31.008
Product last modified at: 2024-11-18T12:45:38.905Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464
  1. Products /
  2. Primary Antibodies /
  3. Phospho-PI3 Kinase Class III (Ser249) Antibody

Phospho-PI3 Kinase Class III (Ser249) Antibody #13857

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Transfected Only
    MW (kDa) 100
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-PI3 Kinase Class III (Ser249) Antibody recognizes transfected levels of PI3 kinase class III protein only when phosphorylated at Ser249.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser249 of human PI3 kinase class III protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Three distinct types of phosphoinositide 3-kinases (PI3K) have been characterized. Unlike other PI3Ks, PI3K class III catalyzes the phosphorylation of phosphatidylinositol at the D3 position, producing phosphatidylinositol-3-phosphate (PIP3) (1). PI3K class III is the mammalian homolog of Vps34, first identified in yeast. PI3K class III interacts with the regular subunit p150, the mammalian homolog of Vps15, which regulates cellular membrane association through myristoylation (2,3). PIP3 recruits several proteins with FYVE or PX domains to membranes regulating vesicular transport and protein sorting (4). Moreover, PI3K class III has been shown to regulate autophagy, trimeric G-protein signaling, and the mTOR nutrient-sensing pathway (5).
    PI3 Kinase Class III/VPS34 is phosphorylated at Ser249 by the autophagy kinase ULK1 (6).

    Database Links

    UniProt ID: Q8NEB9


    Entrez-Gene Id: 5289


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    For Research Use Only. Not For Use In Diagnostic Procedures.
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