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Product last modified at: 2025-01-01T09:01:25.170Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Phospho-PINK1 (Ser228) (E9K3K) Rabbit mAb #89010

Filter:
  • WB
Western Blotting Image 1: Phospho-PINK1 (Ser228) (E9K3K) Rabbit mAb
Western blot analysis of extracts from 293T cells, transfected with a construct expressing Myc-tagged full-length mouse PINK1, untreated (-) or treated with CCCP (10 µM, 8 hr; +), using Phospho-PINK1 (Ser228) (E9K3K) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 89010

Cat. # Size Qty. Price Ships
89010T 20 µl
$187 Jan 7
89010S 100 µl
$431 Jan 7

Supporting Data

REACTIVITY M
SENSITIVITY Transfected Only
MW (kDa) 60, 50
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • M-Mouse 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Simple Western™ 1:50 - 1:250

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Phospho-PINK1 (Ser228) (E9K3K) Rabbit mAb recognizes transfected levels of PINK1 protein only when phosphorylated at Ser228. Non-specific bands of unknown origin are observed migrating at ~32 kDa, and at ~80 kDa in mouse cell lines. Human species reactivity is weak by western blot.

Species Reactivity:

Mouse

The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

Species predicted to react based on 100% sequence homology:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser228 of human PINK1 protein.

Background

PTEN induced putative kinase 1, PINK1, is a mitochondrial serine/threonine kinase involved in the normal function and integrity of mitochondria, as well as in reduction of cytochrome c release from mitochondria (1-3). PINK1 phosphorylates Parkin and promotes its translocation to mitochondria (2). Research studies have shown that mutations in PINK1 are linked to autosomal recessive early onset Parkinson’s disease, and are associated with loss of protective function, mitochondrial dysfunction, aggregation of α-synuclein, as well as proteasome dysfunction (1,3).

It has been shown that autophosphorylation of PINK1 at Ser228 and Ser402 is necessary for Parkin recruitment to damaged mitochondria (4).
For Research Use Only. Not For Use In Diagnostic Procedures.
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