Phospho-PLK1 (Thr210) Antibody #5472
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 62 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
Substitution of Thr210 with Asp has been reported to elevate PLK1 kinase activity and delay/arrest cells in mitosis, while a Ser137Asp substitution leads to S-phase arrest (12). In addition, while DNA damage has been found to inhibit PLK1 kinase activity, the Thr210Asp mutant is resistant to this inhibition (13). PLK1 has been reported to be phosphorylated in vivo at Ser137 and Thr210 in mitosis; DNA damage prevents phosphorylation at these sites (14).
- Nigg, E.A. (1998) Curr Opin Cell Biol 10, 776-83.
- Toyoshima-Morimoto, F. et al. (2002) EMBO Rep 3, 341-8.
- Toyoshima-Morimoto, F. et al. (2001) Nature 410, 215-20.
- Peter, M. et al. (2002) EMBO Rep 3, 551-6.
- Jackman, M. et al. (2003) Nat Cell Biol 5, 143-8.
- Nakajima, H. et al. (2003) J Biol Chem 278, 25277-80.
- Sumara, I. et al. (2002) Mol Cell 9, 515-25.
- Hauf, S. et al. (2001) Science 293, 1320-3.
- Peters, J.M. (1999) Exp. Cell Res. 248, 339-49.
- Kraft, C. et al. (2003) EMBO J 22, 6598-609.
- Kotani, S. et al. (1998) Mol Cell 1, 371-80.
- Jang, Y.J. et al. (2002) J Biol Chem 277, 44115-20.
- Smits, V.A. et al. (2000) Nat Cell Biol 2, 672-6.
- Tsvetkov, L. and Stern, D.F. (2005) Cell Cycle 4, 166-71.
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