Render Target: STATIC
Render Timestamp:
4/4/2025, 7:41:44 AM EDT
4/4/2025, 11:41:44 AM UTC
Commit: c91f970ca8df4f527662a05c7bd6e4d03c6fa173
XML generation date: 2025-03-07 13:07:19.333
Product last modified at: 2024-05-30T07:07:59.925Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Phospho-RIP2 (Ser176) (E1I9J) Rabbit mAb #14397

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  • WB
  • IP

Inquiry Info. # 14397

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    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 61
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-RIP2 (Ser176) (E1I9J) Rabbit mAb recognizes endogenous levels of RIP2 protein only when phosphorylated at Ser176. This antibody also cross-reacts with unidentified proteins of approximately 25 kDa and 38 kDa in some cell lines.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser176 of human RIP2 protein.

    Background

    Receptor Interacting Protein 2 (RIP2) is a serine/threonine kinase with a carboxy-terminal caspase activation and recruitment domain (CARD). Association of RIP2 with the tumor necrosis factor receptor (TNFR) causes activation of NF-κB and induction of apoptosis (1-3). Expression of RIP2 is induced in macrophages upon exposure to bacterial cell wall components, such as LPS. RIP2-deficient mouse models demonstrate that this kinase integrates and transduces signals for both the innate and adaptive immune system (4,5).
    The RIP2 Ser176 residue serves as a regulatory autophosphorylation site and can be used to monitor the activation state of RIP2 (6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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