Phospho-SAMHD1 (Thr592) (D7O2M) Rabbit mAb #89930
- WB
- IP
- F
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 72 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| Flow Cytometry (Fixed/Permeabilized) | 1:800 |
Storage
For a carrier free (BSA and azide free) version of this product see product #27254.
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Phosphorylation of Thr592 by cyclin A2/CDK1 was identified as a regulatory mechanism that controls SAMHD1 activity (7,8). SAMHD1 is phosphorylated in proliferating cells, which inhibits its ability to block HIV infection. In resting cells or in cells treated with PMA (TPA) or IFN-α, SAMHD1 phosphorylation is decreased and cells are refractory to HIV infection (7,8).
- Rice, G.I. et al. (2009) Nat Genet 41, 829-32.
- Laguette, N. et al. (2011) Nature 474, 654-7.
- Hrecka, K. et al. (2011) Nature 474, 658-61.
- Powell, R.D. et al. (2011) J Biol Chem 286, 43596-600.
- Goldstone, D.C. et al. (2011) Nature 480, 379-82.
- Lahouassa, H. et al. (2012) Nat Immunol 13, 223-8.
- Cribier, A. et al. (2013) Cell Rep 3, 1036-43.
- White, T.E. et al. (2013) Cell Host Microbe 13, 441-51.
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