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Phospho-(Ser/Thr) PKA Substrate Antibody

Phospho-(Ser/Thr) PKA Substrate Antibody #9621

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Supporting Data

REACTIVITY	All
SENSITIVITY	Endogenous
MW (kDa)
SOURCE	Rabbit

Application Key:

WB-Western Blotting
IP-Immunoprecipitation
IHC-Immunohistochemistry

Species Cross-Reactivity Key:

All-All Species Expected

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:100
Immunohistochemistry (Paraffin)	1:50 - 1:200
Peptide ELISA (DELFIA)	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Phospho-(Ser/Thr) PKA Substrate Antibody detects peptides and proteins containing a phospho-serine/threonine residue with arginine at the -3 position. It is a useful tool in identifying substrates of AGC family kinases, including PKA and PKC. It does not cross-react with the nonphosphorylated PKA substrate motif.

Species Reactivity:

All Species Expected

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phospho-PKA substrate peptide. Antibodies are purified by protein A and peptide affinity chromatography.

Background

An important class of kinases, refered to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Phospho-PKA substrate-specific antibodies from Cell Signaling Technology are powerful tools for investigating the regulation of phosphorylation by PKA and other Arg-directed kinases, as well as for high throughput kinase drug discovery.

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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