Phospho-SF3B1 (Thr313) (D8D8V) Rabbit mAb #25009
- WB
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 155 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
Phosphorylation of SF3B1 at Thr313 is only found in catalytically active spliceosomes and associates mainly with chromatin, where about 80% of pre-mRNA splicing occurs. Treatment with a transcription inhibitor 5,6-dichloro-1-β-d-ribofuranosylbenzimidazole (DRB) leads to a decreased supply of pre-mRNA, resulting in the loss of phospho-SF3B1 (Thr313), consistent with its association with active splicing (7).
- Jurica, M.S. and Moore, M.J. (2003) Mol Cell 12, 5-14.
- Cazzola, M. et al. (2013) Blood 121, 260-9.
- Bonnal, S. et al. (2012) Nat Rev Drug Discov 11, 847-59.
- Gozani, O. et al. (1998) Mol Cell Biol 18, 4752-60.
- Quesada, V. et al. (2012) Nat Genet 44, 47-52.
- Baliakas, P. et al. (2015) Leukemia 29, 329-36.
- Girard, C. et al. (2012) Nat Commun 3, 994.
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