Phospho-Smad2 (Ser465/467)/ Smad3 (Ser423/425) (D6G10) XP® Rabbit mAb #9510
We recommend the following alternatives
- IF
- F
Inquiry Info. # 9510
Please see our recommended alternatives.
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Immunocytochemistry) | 1:200 |
| Flow Cytometry (Fixed/Permeabilized) | 1:400 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
Following stimulation by TGF-β, Smad2 and Smad3 become phosphorylated at their carboxyl termini (serines 465 and 467 on Smad2; serines 423 and 425 on Smad3) by the receptor kinase TGF-β R1(9-11). Following phosphorylation, Smad2 and Smad3 form a heteromeric complex with the co-smad family member Smad4. These complexes are translocated to the nucleus where they bind DNA and regulate gene transcription.
- Heldin, C.H. et al. (1997) Nature 390, 465-71.
- Attisano, L. and Wrana, J.L. (1998) Curr Opin Cell Biol 10, 188-94.
- Derynck, R. et al. (1998) Cell 95, 737-40.
- Massagué, J. (1998) Annu Rev Biochem 67, 753-91.
- Whitman, M. (1998) Genes Dev 12, 2445-62.
- Wrana, J.L. (2000) Sci STKE 2000, re1.
- Attisano, L. and Wrana, J.L. (2002) Science 296, 1646-7.
- Moustakas, A. et al. (2001) J Cell Sci 114, 4359-69.
- Abdollah, S. et al. (1997) J. Biol. Chem. 272, 27678-27685.
- Souchelnytskyi, S. et al. (1997) J. Biol. Chem. 272, 28107-28115.
- Liu, X. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 10669-10674.
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