Phospho-SUMO-1 (Ser2) (F9B6U) Rabbit mAb #88855
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Serine 2 of SUMO-1 was shown to be phosphorylated in cells using mass spectrometry analyses (12). This phosphorylation site was also identified in yeast, Drosophila, and Arabidopsis, suggesting an evolutionary conserved function for this modification (12,13).
- Schwartz, D.C. and Hochstrasser, M. (2003) Trends Biochem. Sci. 28, 321-8.
- Kim, K.I. et al. (2002) J. Cell Physiol. 191, 257-68.
- Matunis, M.J. et al. (1996) J. Cell Biol. 135, 1457-70.
- Duprez, E. et al. (1999) J. Cell Sci. 112, 381-93.
- Gostissa, M. et al. (1999) EMBO J. 18, 6462-74.
- Rodriguez, M.S. et al. (1999) EMBO J. 18, 6455-61.
- Desterro, J.M. et al. (1998) Mol. Cell 2, 233-9.
- Tatham, M.H. et al. (2001) J. Biol. Chem. 276, 35368-74.
- Azuma, Y. et al. (2003) J. Cell Biol. 163, 477-87.
- Li, Y. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 259-64.
- Saitoh, H. and Hinchey, J. (2000) J. Biol. Chem. 275, 6252-8.
- Matic, I. et al. (2008) J Proteome Res 7, 4050-7.
- Roitinger, E. et al. (2015) Mol Cell Proteomics 14, 556-71.
Limited Uses
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