Phospho-TFE3 (Ser321) (F1E8R) Rabbit mAb #38274
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 76-90 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Phosphorylation of TFE3 at Ser321 by mTOR leads to cytoplasmic retention analogous to Ser211 on TFEB (6). Dephosphorylation of TFEB and TFE3 by protein phosphatase 2A in response to oxidative stress leads to their nuclear translocation and activation (7).
- Miller, A.J. et al. (2005) J Biol Chem 280, 146-55.
- Martina, J.A. et al. (2014) Cell Mol Life Sci 71, 2483-97.
- Martina, J.A. et al. (2014) Sci Signal 7, ra9.
- Betschinger, J. et al. (2013) Cell 153, 335-47.
- Kauffman, E.C. et al. (2014) Nat Rev Urol 11, 465-75.
- Wada, S. et al. (2016) Genes Dev 30, 2551-2564.
- Martina, J.A. and Puertollano, R. (2018) J Biol Chem 293, 12525-12534.
Limited Uses
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