Render Target: STATIC
Render Timestamp: 2024-12-20T12:23:30.256Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:56:00.818
Product last modified at: 2024-12-17T18:57:46.517Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Phospho-Thr-Pro-Pro Motif [pTPP] (D61C3) Rabbit mAb #5757

Filter:
  • WB

    Supporting Data

    REACTIVITY All
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • All-All Species Expected 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-Thr-Pro-Pro Motif [pTPP] (D61C3) Rabbit mAb recognizes endogenous levels of proteins only when phosphorylated within the TPP motif. It does not cross react with phospho-serine or phospho-threonine in other contexts.

    Species Reactivity:

    All Species Expected

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with synthetic peptide library containing the phospho-Thr-Pro-Pro motif.

    Background

    The phospho-Thr-Pro-Pro motif is a subgroup of the phospho-Thr-Pro motif that is phosphorylated by proline-directed protein kinases, including MAP kinases and cyclin dependent kinases (CDKs). Proline-directed phosphorylation is one of the major regulatory phosphorylation events in cell proliferation, cell differentiation, and a number of other essential cellular processes (1-6). This motif was identified in both phospho-proteomic and motif X analyses as a significant phospho-thr motif (7,8).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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