Render Target: STATIC
Render Timestamp: 2024-11-21T14:00:26.714Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-15 12:38:28.097
Product last modified at: 2024-09-30T08:02:34.255Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Phospho-TPOR (Tyr626) (D3H7B) Rabbit mAb #12712

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 85-90
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-TPOR (Y626) (D3H7B) Rabbit mAb recognizes endogenous levels of TPOR protein only when phosphorylated at Tyr626. This antibody cross-reacts with a 30 kDa protein of unknown origin. This antibody may cross-react with some tyrosine-phosphorylated proteins including the EGF Receptor, ROS1, and FLT3.

    Species Reactivity:

    Human

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Mouse, Rat, Monkey, Bovine

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr626 of human TPOR protein.

    Background

    Thrombopoietin receptor (TPOR, c-Mpl) is a hematopoietic receptor that binds the growth factor, thrombopoietin (TPO), responsible for regulation of platelet production (1-3). Expression of TPOR by megakaryocytes is required for megakaryocyte growth and development (4). TPOR is also expressed by hematopoietic stem cells and is required for stem cell maintenance and expansion (5). Studies show that mice lacking either TPOR or TPO have severely reduced numbers of platelets and megakaryocytes as well as decreased numbers of other hematopoietic lineages (4,5). Binding of TPO to TPOR induces receptor dimerization that leads to phosphorylation and activation of the tyrosine kinase Jak2. Activated Jak2 associates with the cytoplasmic domain of TPOR (6,7) and phosphorylates TPOR at Tyr626 and Tyr631 (8). These phosphorylated tyrosine residues provide docking sites for downstream signaling molecules including Stat3, Stat5, Shc, and SHIP (7-9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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