Phospho-TWIST1 (Ser68) (E8I6K) Rabbit mAb #42444
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 26 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
Serine 68 was identified as a major phosphorylation site of TWIST1 and shown to be regulated by mitogen-activated protein kinases (MAPKs) to ultimately stabilize the protein and promote invasiveness in breast cancer cells (9).
- Thisse, B. et al. (1987) Nucleic Acids Res. 15, 3439-53.
- Lee, M.S. et al. (1999) J. Cell Biochem. 75, 566-77.
- Chen, Z.F. and Behringer, R.R. (1995) Genes Dev. 9, 686-99.
- Howard, T.D. et al. (1997) Nat. Genet. 15, 36-41.
- Connerney, J. et al. (2006) Dev. Dyn. 235, 1345-57.
- Watanabe, O. et al. (2004) Anticancer Res. 24, 3851-6.
- Yang, J. et al. (2004) Cell 117, 927-39.
- Wang, X. et al. (2004) Oncogene 23, 474-82.
- Hong, J. et al. (2011) Cancer Res 71, 3980-90.
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