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Phospho-Tyrosine (4G10) Mouse mAb

Phospho-Tyrosine (4G10) Mouse mAb #96215

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Supporting Data

REACTIVITY	All
SENSITIVITY	Endogenous
MW (kDa)
Source/Isotype	Mouse IgG2b

Application Key:

WB-Western Blotting
IP-Immunoprecipitation
IHC-Immunohistochemistry

Species Cross-Reactivity Key:

All-All Species Expected

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:100
Immunohistochemistry (Paraffin)	1:1600 - 1:6400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. This product is stable for 36 months when stored at -20C.

Protocol

Specificity / Sensitivity

Phospho-Tyrosine (4G10) Mouse mAb recognizes a broad range of tyrosine-phosphorylated proteins and peptides. This antibody does not cross-react with proteins or peptides containing phospho-Ser or phospho-Thr residues.

Species Reactivity:

All Species Expected

Source / Purification

Monoclonal antibody is produced by immunizing animals with phosphotyramine-KLH.

Background

Tyrosine phosphorylation plays a key role in cellular signaling (1). Research studies have shown that in cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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