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XML generation date: 2024-12-25 01:37:34.816
Product last modified at: 2025-01-01T09:01:33.720Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Phospho-Tyrosine (9E9) Mouse mAb (BSA and Azide Free) #80941

Filter:
  • ELISA
ELISA Image 1: Phospho-Tyrosine (9E9) Mouse mAb (BSA and Azide Free)
Data using Phospho-HER2/ErbB2 (panTyr) Matched Antibody Pair #99159 are shown. The two antibodies function as a capture-target-detection sandwich (#19783 and #80941, respectively) to detect endogenous protein expression in positive and negative controls.

To Purchase # 80941

Cat. #

Size

80941SF
100 µg

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa)
Source/Isotype Mouse IgG2a kappa
Application Key:
  • ELISA-ELISA 
Species Cross-Reactivity Key:
  • H-Human 

Product Information

Product Usage Information

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

Formulation

Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

Phospho-Tyrosine (9E9) Mouse mAb (BSA and Azide Free) recognizes a broad range of tyrosine-phosphorylated proteins.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with phospho-tyrosine containing peptides.

Background

Tyrosine phosphorylation plays a key role in cellular signaling (1). Research studies have shown that in cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.
For Research Use Only. Not For Use In Diagnostic Procedures.
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