Phospho-Ubiquitin (Ser57) (F1I4R) Rabbit mAb #88056
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Transfected Only |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Regulation of ubiquitin homeostasis is mediated by phosphorylation at Ser57, with mutation at Ser57 conferring increased rates of endocytic trafficking and ubiquitin turnover (8). Moreover, phosphorylation of Ser57 has been shown to function in the oxidative stress response in Saccharomyces cerevisiae (9). In vitro kinase assays have demonstrated that kinases in the MARK and SIK families can phosphorylate ubiquitin at Ser57 (9).
- Ciechanover, A. (1998) EMBO J 17, 7151-60.
- Hochstrasser, M. (2000) Nat Cell Biol 2, E153-7.
- Hochstrasser, M. (2000) Science 289, 563-4.
- Bernardi, R. et al. (2000) Oncogene 19, 2447-54.
- Aberle, H. et al. (1997) EMBO J 16, 3797-804.
- Salomoni, P. and Pandolfi, P.P. (2002) Nat Cell Biol 4, E152-3.
- Jesenberger, V. and Jentsch, S. (2002) Nat Rev Mol Cell Biol 3, 112-21.
- Lee, S. et al. (2017) Elife 6, e29176. doi: 10.7554/eLife.29176.
- Hepowit, N.L. et al. (2020) Elife 9, e58155. doi: 10.7554/eLife.58155.
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