Phospho-YAP (Ser397) (D1E7Y) Rabbit mAb #13619
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 65-78 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Phosphorylation of YAP at Ser397 (annotated as Ser381 by Zhao et al. 2010) primes YAP for subsequent phosphorylation by CK1δ/ε in an adjacent phosphodegron (9). Upon phosphorylation, the phosphodegron recruits the β-TrCP (SCF) ubiquitin ligase complex, which ubiquitinates YAP to trigger its proteolytic degradation in the proteasome.
- Sudol, M. (1994) Oncogene 9, 2145-52.
- Mohler, P.J. et al. (1999) J Cell Biol 147, 879-90.
- Espanel, X. and Sudol, M. (2001) J Biol Chem 276, 14514-23.
- Sudol, M. et al. (1995) FEBS Lett 369, 67-71.
- Yagi, R. et al. (1999) EMBO J 18, 2551-62.
- Dong, J. et al. (2007) Cell 130, 1120-33.
- Zhao, B. et al. (2010) Genes Dev 24, 862-74.
- Zhao, B. et al. (2007) Genes Dev 21, 2747-61.
- Yu, F.X. et al. (2012) Cell 150, 780-91.
- Zhao, B. et al. (2010) Genes Dev 24, 72-85.
- Zhao, B. et al. (2010) Genes Dev 24, 72-85.
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