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Product last modified at: 2025-02-13T12:45:13.853Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

PIAS3 Antibody #4164

Filter:
  • WB
Western Blotting Image 1: PIAS3 Antibody
Western blot analyis of extracts from various cell lines, including COS-7 cells, mock transfected or transfected with mouse PIAS1 or PIAS3, using PIAS3 Antibody. Overexpressed PIAS3 runs as a doublet as previously reported (15).

To Purchase # 4164

Cat. # Size Qty. Price
4164S 100 µl
$306

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 65-75
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

PIAS3 Antibody detects endogenous levels of total PIAS3 protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to Thr585 of human PIAS3. Antibodies were purified by protein A and peptide affinity chromatography.

Background

The protein inhibitor of activated Stat (PIAS) proteins, which include PIAS1, PIAS3, PIASx, and PIASy, were originally characterized based on their interaction with the Stat family of transcription factors (1,2). PIAS1, PIAS3, and PIASx interact with and repress Stat1, Stat3, and Stat4, respectively (1-3). Deletion of PIAS1 leads to inhibition of interferon-inducible genes and increased protection against infection (4). The PIAS family contains a conserved RING domain that has been linked to a function as a small ubiquitin-related modifier (SUMO) ligase, coupling the SUMO conjugating enzyme Ubc9 with its substrate proteins (5,6). Numerous studies have now shown that PIAS family members can regulate the activity of transcription factors through distinct mechanisms, including NF-κB (7,8), c-Jun, p53 (5,9), Oct-4 (10), and Smads (11,12). The activity of PIAS1 is regulated by both phosphorylation and arginine methylation. Inflammatory stimuli can induce IKK-mediated phosphorylation of PIAS1 at Ser90, which is required for its activity (13). In addition, PRMT1 induces arginine methylation of PIAS1 at Arg303 following interferon treatment and is associated with its repressive activity on Stat1 (14).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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