Render Target: STATIC
Render Timestamp: 2024-12-26T11:08:51.741Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-12-03 23:01:07.840
Product last modified at: 2024-12-10T09:00:39.095Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Piezo1 (F1T5B) Rabbit mAb #62649

Filter:
  • WB

    Supporting Data

    REACTIVITY H R
    SENSITIVITY Endogenous
    MW (kDa) 300-350, 250
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Piezo1 (F1T5B) Rabbit mAb recognizes endogenous levels of total Piezo1 protein. This antibody cross-reacts with a band of unknown origin at approximately 70 kDa by western blot.

    Species Reactivity:

    Human, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr1569 of human Piezo1 protein.

    Background

    Piezo1 is a multi-pass transmembrane protein that serves as a mechanosensitive cationic ion channel, directly activated by membrane stretch (1,2). Piezo1 protein is a trimer with three blade-like propellers inserted within the lipid bilayer with a central ion channel that senses various mechanical forces such as membrane tension, shear stress, and cyclical pressure (3,4). Membrane stress induces Piezo1 conformational change, leading to ion pore opening and Ca2+ influx, which further activates multiple related signaling pathways such as CaMKII, NFκB, and Akt (5,6). The protein is engaged in a variety of essential biological processes, including vascular and lymphatic valve development (7,8), musculoskeletal function (9), and bone formation (10). Piezo1 protein plays a regulatory role in macrophage-mediated inflammatory diseases, atherosclerosis, and cancer metastasis (11-14).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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