Render Target: STATIC
Render Timestamp: 2024-12-26T11:12:45.827Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:58:57.130
Product last modified at: 2024-12-18T22:45:10.150Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

PIKFYVE (E4X3R) Rabbit mAb #92839

Filter:
  • WB

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 250
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PIKFYVE (E4X3R) Rabbit mAb recognizes endogenous levels of total PIKFYVE protein.

    Species Reactivity:

    Human, Mouse

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr1653 of human PIKFYVE protein.

    Background

    PIKFYVE is a lipid kinase that phosphorylates position 5 of PIP (phosphatidylinositol) or PI(3)P (phosphatidylinositol 3-phosphate) to form PI5P or PI(3,5)P2 (1,2). The normal cellular levels of the latter two lipids are important in maintaining the functional responses of endosomes, lysosomes, and autophagosomes, as well as the regulation of cell cycle, stress response, T cell activation, and chromatin remodeling (3,4). PIKFYVE functions within a regulatory complex including the PIKFYVE kinase, a scaffold protein Vac14, and a lipid phosphatase Fig4 (Sac) (5,6). The complex localizes to endosomes and lysosomes, regulating endosome-to-TGN retrograde transport, lysosome fission, and lysosomal related autophagy (7-10). Targeted disruption of the PIKFYVE gene is embryonic lethal, indicating the vital role of the kinase in early embryonic development (11). Inhibition of PIKFYVE disrupts lysosome function in autophagy and can selectively kill certain cancer cells (12,13). PIKFYVE inhibition also potentiates immune response to checkpoint blockade in the tumor microenvironment (14).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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