Pim-2 (D1D2) Rabbit mAb #4730
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 40, 38, 34 |
| Source/Isotype | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Pim-2 is highly homologous to Pim-1 with similar oncogenic functions (13,14). Three isoforms of Pim-2 can be generated from alternative start sites which run at 34, 38, and 40 kDa (13). Pim-2 leads to resistance to a variety of apoptotic stimuli and its expression is negatively regulated by growth factor withdrawal (15,16). Increased levels of Pim-2 have also been observed in certain cancers (17,18).
- Mikkers, H. et al. (2004) Mol Cell Biol 24, 6104-15.
- Selten, G. et al. (1986) Cell 46, 603-11.
- Meeker, T.C. et al. (1987) J Cell Biochem 35, 105-12.
- Dautry, F. et al. (1988) J Biol Chem 263, 17615-20.
- Möröy, T. et al. (1993) Proc Natl Acad Sci USA 90, 10734-8.
- Lilly, M. and Kraft, A. (1997) Cancer Res 57, 5348-55.
- Leverson, J.D. et al. (1998) Mol Cell 2, 417-25.
- Winn, L.M. et al. (2003) Cell Cycle 2, 258-62.
- Pasqualucci, L. et al. (2001) Nature 412, 341-6.
- Kim, O. et al. (2004) Oncogene 23, 1838-44.
- Aho, T.L. et al. (2004) FEBS Lett 571, 43-9.
- Yan, B. et al. (2003) J Biol Chem 278, 45358-67.
- van der Lugt, N.M. et al. (1995) EMBO J 14, 2536-44.
- Breuer, M.L. et al. (1989) EMBO J 8, 743-8.
- Fox, C.J. et al. (2003) Genes Dev 17, 1841-54.
- White, E. (2003) Genes Dev 17, 1813-6.
- Cohen, A.M. et al. (2004) Leuk Lymphoma 45, 951-5.
- Dai, H. et al. (2005) Prostate 65, 276-86.
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