Render Target: STATIC
Render Timestamp: 2024-11-22T11:22:08.962Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:57:13.901
Product last modified at: 2024-09-30T08:00:57.635Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Pirin (1E8) Rat mAb #9777

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R Hm Mk B
    SENSITIVITY Endogenous
    MW (kDa) 32
    Source/Isotype Rat IgG2a
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Hm-Hamster 
    • Mk-Monkey 
    • B-Bovine 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Pirin (1E8) Rat mAb detects endogenous levels of Pirin protein. This antibody does not cross-react with other proteins in the cupin superfamily.

    Species Reactivity:

    Human, Mouse, Rat, Hamster, Monkey, Bovine

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with the full-length Pirin protein.

    Background

    Pirin is a highly conserved nuclear protein and a member of the cupin superfamily of proteins, all of which contain two conserved β-barrel fold domains (1). Pirin functions as a co-factor for NFI/CTF1 and Bcl-3, implicating it in DNA replication, transcriptional activation and apoptosis (2,3). Both human and bacterial pirins catalyze the di-oxygenation of quercetin, one of a class of widespread naturally occurring flavenoid compounds that have anti-inflammatory and anti-cancer activities (4). Flavenoids exert these beneficial activities by functioning as antioxidants that stabilize cellular free radical molecules and by directly modulating cell signaling pathways involving PI 3-kinase, Akt/PKB, PKC and MAP kinases (5). Quercetin has also been directly implicated in the regulation of NF-κB activity; thus, Pirin may exert its apoptotic functions both by directly regulating Bcl-3/NF-κB activity and by modulating quercetin levels in the cell (6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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