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PKD/PKCμ Antibody #2052

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Filter:
  • WB
  • F
Western blot analysis of extracts from NIH/3T3 cells, untreated, PDGF-treated (50 ng/ml) or TPA-treated (0.2 µM), using PKD/PKCmu Antibody.
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Inquiry Info. # 2052

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Product Specifications

REACTIVITY H M Mk
SENSITIVITY Endogenous
MW (kDa) 115
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • Mk-Monkey 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Flow Cytometry (Fixed/Permeabilized) 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

PKD/PKCμ Antibody detects endogenous levels of total PKD1/PKCmu protein. This antibody may also react with isoform PKD2, in some species.

Species Reactivity:

Human, Mouse, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser916 of mouse PKD. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Activation of PKC is one of the earliest events in a cascade leading to a variety of cellular responses, such as secretion, gene expression, proliferation, and muscle contraction (1,2). Protein kinase D (PKD), also called PKCμ, is a serine/threonine kinase whose activation is dependent on the phosphorylation of two activation loop sites, Ser744 and Ser748, via a PKC-dependent signaling pathway (3-5). In addition to the two activation loop sites, the carboxy-terminal Ser916 has been identified as an autophosphorylation site for PKD/PKCμ. Phosphorylation at Ser916 correlates with PKD/PKCμ catalytic activity (6).

Alternate Names

PKC; PKC-mu; PKD; PKD-PKC-mu

Pathways

Explore pathways related to this product.


For Research Use Only. Not for Use in Diagnostic Procedures.
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