Render Target: STATIC
Render Timestamp: 2024-11-21T13:25:11.110Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:54:58.123
Product last modified at: 2024-11-15T20:00:08.396Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

PKG-1 (C8A4) Rabbit mAb #3248

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 78
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PKG-1 (C8A4) Rabbit mAb detects endogenous levels of total PKG-1 protein, including both α and β isoforms. The antibody does not cross-react with PKG-2.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to cental residues of human PKG-1.

    Background

    Cyclic GMP-dependent kinases (cGK/PKG) belong to the AGC family of serine/threonine protein kinases. In mammals, two genes encode PKG-1 and PKG-2. Alternative PKG-1 splicing yields α and β isoforms, which display tissue-specific expression patterns in humans (1). All PKG family members are activated by increased cellular cGMP, which binds to the enzyme's regulatory domain inducing a conformational change and leading to enzyme activation. cGMP levels are increased through the activation of guanylyl cyclases, a process known to occur in part through nitric oxide (NO) signaling (2).
    In addition to well established roles in platelet activation and smooth muscle relaxation (3), PKG signaling is important in many biological processes including cardiac contractility, axon guidance, bone growth, contraction of intestinal smooth muscle, and erectile dysfunction (4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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