Render Target: STATIC
Render Timestamp: 2024-12-20T11:05:08.391Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:54:18.627
Product last modified at: 2024-12-17T18:49:11.307Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

PLK2 (D5R2B) Rabbit mAb #14812

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 70
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PLK2 (D5R2B) Rabbit mAb recognizes endogenous levels of total PLK2 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro67 of human PLK2 protein.

    Background

    At least five distinct polo-like kinases exist in mammalian cells: PLK1, PLK2, PLK3, PLK4/SAK, and the non-catalytic PLK5 protein (1). The p53-induced PLK2 functions in centriole duplication, as well as at spindle and S phase checkpoints (3-5). Research studies show that PLK2 expression is related to chemosensitivity in ovarian cancer. Downregulation of PLK2 expression in chemosensitive ovarian cancer cells is associated with a greater chance of relapse in patients following specific treatment regimens (6). PLK2 can phosphorylate α-synuclein at Ser129, which is a site shown to be involved in diseases of the central nervous system (7,8). Polo-like kinase 2 also phosphorylates GEFs and GAPs, regulating Ras and Rap small GTPase function in neurons (9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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