PLVAP (E3X9D) Rabbit mAb (BSA and Azide Free) #64266

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Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	70, 140
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IHC-Immunohistochemistry
IF-Immunofluorescence
F-Flow Cytometry

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

This product is the carrier free version of product #38238. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

Formulation

Supplied in 1X PBS (10 mM Na₂HPO₄, 3 mM KCl, 2 mM KH₂PO₄, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

For standard formulation of this product see product #38238

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

PLVAP (E3X9D) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total PLVAP protein. Non-specific staining was observed in gall bladder by immunohistochemistry.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PLVAP protein.

Background

PLVAP (plasmalemma vesicle-associated protein) is an endothelial cell (EC)-specific type II membrane protein. It forms diaphragms that bridge the opening of caveolae, fenestrae, and trans-endothelial channels and plays a role in regulating vascular permeability. PLVAP expression is restricted to a subset of ECs in the normal microvasculature, but it is not expressed in the ECs of large vessels, with the exception of the endocardial lining of the heart chambers (1). While PLVAP is expressed in ECs in the brain and retina during embryogenesis and postnatal development, it is not expressed in mature endothelium that forms blood–brain barrier (BBB) and blood–retinal barrier (BRB). However, under pathological conditions associated with barrier disruption such as brain ischemia, cancer, and diabetic retinopathy, PLVAP expression is induced in endothelium of BBB and BRB (2). PLVAP was previously considered as an EC marker of the vascular system, its expression is now reported in lymphatic ECs (LECs) in lymph nodes and it may play a role in regulating the parenchymal entry of lymphocytes and soluble antigens (3,4).

Database Links

UniProt ID: Q9BX97

Entrez-Gene Id: 83483

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For Research Use Only. Not For Use In Diagnostic Procedures.

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