Render Target: STATIC
Render Timestamp: 2024-12-27T11:29:39.592Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-20 06:19:21.927
Product last modified at: 2024-11-07T20:45:09.839Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

PLXND1 Antibody #92470

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 250
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PLXND1 Antibody recognizes endogenous levels of total PLXND1 protein.

    Species Reactivity:

    Human

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Rat

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val1665 of human PLXND1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    PLXND1 (PlexinD1) is a type I transmembrane receptor for semaphorin (SEMA) family signaling molecules (1). PLXND1 has an extracellular SEMA binding domain, and a cytoplasmic tail containing RasGAP motifs and a RhoGTPase-binding domain. Upon ligand binding, PLXND1 undergoes conformational change and acquires GAP activity that inactivates downstream Rac/Ras signaling, leading to focal adhesion destabilization (2). The PLXND1 signaling pathway plays important roles in neuronal synapse formation, vascular branching, and thymocyte migration (2-4). Increased expression of PLXND1 is positively correlated with tumor stages in multiple cancer types (6). This is supported by gene knockdown experiments that suggest that SEMA/PLXND1 signaling may contribute metastatic progression (7-8).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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