Render Target: STATIC
Render Timestamp: 2025-01-22T14:41:41.244Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-09-17 16:01:11.355
Product last modified at: 2025-01-01T09:02:03.551Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

PMS2 (M0R4G) Mouse mAb #78576

Filter:
  • IHC

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Mouse IgG1
    Application Key:
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    IHC Leica Bond 1:200 - 1:800
    Immunohistochemistry (Paraffin) 1:200 - 1:800

    Storage

    Supplied as liquid tissue culture supernatant containing sodium azide as a preservative. Stable for 12 months when stored at 4°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PMS2 (M0R4G) Mouse mAb recognizes endogenous levels of PMS2 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a prokaryotic recombinant protein corresponding to 160 amino acids of the C–terminal region of human PMS2 protein.

    Background

    DNA mismatch repair (MMR), a conserved process for detecting and correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity (1). In prokaryotes, a MutS homodimer recruits a MutL homodimer to sites of DNA mismatches. In eukaryotes, six MutS homologues (MSH1-6) and four MutL homologues (MLH1, PMS2, PMS1, and MLH3) have been identified. Heterodimers composed of two MutL homologues detect distinct DNA mismatch lesions, and heterodimers composed of two MutS homologues perform the repair (2).
    Microsatellite instability (MSI) is a predisposition to genetic mutation resulting from MMR deficiency (dMMR). High MSI (MSI-H) arising from dMMR results in Lynch syndrome, also known as hereditary non-polyposis colorectal cancer (HNPCC). Lynch syndrome is associated with colon cancer, as well as other human cancers (3). MSI and dMMR are strongly associated with tumor responsiveness to immune checkpoint blockade (4,5). MSI status can be determined through PCR amplification of microsatellite markers and/or immunohistochemical detection of MMR proteins MLH1, PMS2, MSH2, and MSH6. The absence of expression of any of these MMR proteins indicates dMMR (3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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