Render Target: STATIC
Render Timestamp: 2024-11-12T10:28:59.145Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-09-30 01:57:55.970
Product last modified at: 2024-09-30T08:01:14.306Z
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

PODXL (E8O1S) Rabbit mAb #55601

Filter:
  • WB
  • IP
  • IHC
  • IF

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 200
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:200
    Immunohistochemistry (Paraffin) 1:200 - 1:800
    Immunofluorescence (Immunocytochemistry) 1:200 - 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #66798.

    Protocol

    Specificity / Sensitivity

    PODXL (E8O1S) Rabbit mAb recognizes endogenous levels of total PODXL protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human PODXL protein.

    Background

    Podocalyxin is an O-glycosylated and sialylated membrane protein that is closely similar to CD34, the well characterized marker for hematopoietic stem cells (1). In association with several intracellular binding partners, podocalyxin contributes to adhesion and cell morphology (2-4). Podocalyxin is expressed in hematopoietic tissues during stages of active hematopoietic expansion in embryonic development and in more restricted, mainly megakaryocytic lineages in the adult (1,4). Expression also occurs in kidney podocytes, both in embryo and adult, where podocalyxin is essential for organ development and proper kidney function (5-7). A few other cell types that express podocalyxin include some specialized vascular endothelia (8,9) and a subset of neurons (10). It is also aberrantly expressed in a diverse number of cancers, including breast, testicular, pancreatic cancers, and in leukemia (11). Expression is often correlated with poor outcome (11).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    XP is a registered trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.