Poly/Mono-ADP Ribose (D9P7Z) Rabbit mAb (BSA and Azide Free) #29889
- WB
- IF
Supporting Data
| REACTIVITY | All |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Storage
Specificity / Sensitivity
Poly/Mono-ADP Ribose (D9P7Z) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of ADP ribosylated proteins and does not cross-react with other post-translational modifications.
Species Reactivity:
All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with KLH modified on lysines with ADP ribose.
Background
ADP-ribosylation is involved in a variety of cellular processes, including mitotic spindle formation, chromatin decondensation, cell stress response, retroviral silencing, RNA biology, and transcription, but the most well-known function of ADP-ribose chains is to serve as a scaffold for recruiting DNA repair proteins that contain PAR-binding modules to sites of DNA damage (6). X-ray repair cross-complementing protein 1 (XRCC1), histone macroH2A1, the E3 ubiquitin ligase RNF146 (Iduna), and many of the PARPs themselves, among others, contain PAR-binding motifs (PBMs) or domains: WWE, PAR-binding zinc-finger (PBZ), or macrodomains (7). PARylation has a central role in cell survival, and is tightly regulated. PARP deficiency can leave a cell vulnerable to DNA damage-induced apoptosis, while hyper PARylation can lead to parthanatos, a unique form of cell death (8). The role of PARylation in DNA repair has inspired great interest in developing candidate drug inhibitors for PARP, in particular to treat breast, prostate, and small cell lung cancers with mutations in DNA repair genes like BRCA1/2, CHK2, or ATM. Stat1, PERK, p53, G-actin, and Ras are just a few examples of proteins that are functionally modulated by ADP-ribosylation (6,7). Modification by ADP-ribose can block protein interactions or, in the case of P2X7, cause a conformational change that, in the presence of ART2 expression, sensitizes naive murine T-cells to extracellular NAD+, leading to apoptosis (9).
- Koch-Nolte, F. et al. (2008) Front Biosci 13, 6716-29.
- Leung, A.K. (2014) J Cell Biol 205, 613-9.
- Laing, S. et al. (2011) Amino Acids 41, 257-69.
- Vyas, S. et al. (2014) Nat Commun 5, 4426.
- Vivelo, C.A. and Leung, A.K. (2015) Proteomics 15, 203-17.
- Gupte, R. et al. (2017) Genes Dev 31, 101-126.
- Wei, H. and Yu, X. (2016) Genomics Proteomics Bioinformatics 14, 131-139.
- David, K.K. et al. (2009) Front Biosci (Landmark Ed) 14, 1116-28.
- Seman, M. et al. (2003) Immunity 19, 571-82.
Limited Uses
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