Pontin/RUVBL1 Antibody #12300
- WB
Supporting Data
| REACTIVITY | H M R Hm Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 50 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Hm-Hamster
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
A number of research studies have reported elevated levels of Pontin in selected cancer subtypes, including hepatocellular carcinoma and colon cancer (8-10). Interestingly, Pontin was reported to potentiate the TCF/LEF1-β-catenin transcriptional complex, whereas Reptin was antagonistic to the same complex, highlighting their related, but non-redundant, function (5). Research studies also suggest a functional role for Pontin and Reptin in the DNA damage response. Both proteins are phosphorylated by ATM and Rad3-related following DNA damage (11), while Pontin is reportedly required for TIP60 activity during the DNA damage response (12).
- Jha, S. and Dutta, A. (2009) Mol Cell 34, 521-33.
- Gallant, P. (2007) Trends Cell Biol 17, 187-92.
- Huber, O. et al. (2008) Cancer Res 68, 6873-6.
- Kim, J.H. et al. (2005) Nature 434, 921-6.
- Bauer, A. et al. (2000) EMBO J 19, 6121-30.
- Wood, M.A. et al. (2000) Mol Cell 5, 321-30.
- Dugan, K.A. et al. (2002) Oncogene 21, 5835-43.
- Blanc, J.F. et al. (2005) Proteomics 5, 3778-89.
- Lauscher, J.C. et al. (2007) Hum Pathol 38, 978-85.
- Huber, O. et al. (2008) Cancer Res 68, 6873-6.
- Matsuoka, S. et al. (2007) Science 316, 1160-6.
- Jha, S. et al. (2008) Mol Cell Biol 28, 2690-700.
Limited Uses
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