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POU2F3 Antibody #92579

Filter:
  • WB
  • IP
Western blot analysis of extracts from NCI-H526, MDA-MB-453, and HCT 116 cells using POU2F3 Antibody (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Expression levels of POU2F3 among cell lines are consistent with expectations based on publicly available bioinformatic databases, confirming specificity of the antibody for POU2F3.

To Purchase # 92579

Cat. # Size Qty. Price
92579S 100 µl
$306

Product Specifications

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 45-60
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

POU2F3 Antibody recognizes endogenous levels of total POU2F3 protein.

Species Reactivity:

Human

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu160 of human POU2F3 protein. Antibodies are purified by peptide affinity chromatography.

Background

POU2F3 (also known as OCT-11 and SKN-1) belongs to the OCT family of octamer motif-binding transcription factors, members of which play varied, diverse roles in animal development. POU2F3 is a lineage-defining transcription factor, responsible for driving differentiation of specialized chemosensory cells in the gastrointestinal and respiratory tracts (1-3). Tuft cells are a subtype of chemosensory cell, defined by POU2F3 expression, and which are hypothesized to be the origin of a distinct, non-neuroendocrine subtype of small cell lung cancer (SCLC) known as tuft cell-like SCLC (4). Consistent with its lineage-defining role in tuft cell development, POU2F3 was identified through a Crispr/Cas9 screen as a master regulator of tuft cell-like SCLC (5).
For Research Use Only. Not for Use in Diagnostic Procedures.
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