Render Target: STATIC
Render Timestamp: 2025-01-03T12:16:17.630Z
Commit: 286c369131ceeedcf44c821941824d8d7e009e57
XML generation date: 2024-08-01 15:23:24.086
Product last modified at: 2025-01-01T09:04:41.982Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

PP1α Antibody #2582

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R Mk Dm
    SENSITIVITY Endogenous
    MW (kDa) 38
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 
    • Dm-D. melanogaster 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PP1α Antibody detects endogenous levels of total PP1α protein. This antibody detects recombinant levels of PP1γ and does not cross-react with PP1δ.

    Species Reactivity:

    Human, Mouse, Rat, Monkey, D. melanogaster

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human PP1alpha. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Type 1 protein phosphatase (PP1), a serine/threonine phosphatase, is highly conserved in eukaryotic cells. Four isoforms of PP1 have been characterized: PP1α, PP1δ, PP1γ1 and PP1γ2 (1). Involvement in cell cycle regulation is one of the biological functions of PP1 (1). It has been illustrated that PP1 dephosphorylates Rb and cdc25 during mitosis (2,3). A cell cycle-dependent phosphorylation at Thr320 of PP1α by cdc2 kinase inhibits PP1α activity (4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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