Render Target: STATIC
Render Timestamp: 2024-12-20T11:39:35.998Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:58:13.077
Product last modified at: 2024-12-17T19:00:39.321Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

PPARδ (E8O3H) Rabbit mAb #74076

Filter:
  • WB
  • IP
  • ChIP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 50
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • ChIP-Chromatin Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100
    Chromatin IP 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PPARδ (E8O3H) Rabbit mAb recognizes endogenous levels of total PPARδ protein. This antibody may cross-react with overexpressed PPARα protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment specific to human PPARδ protein.

    Background

    Peroxisome proliferator-activated receptor-δ (PPARδ, also known as PPARβ or PPARβ/δ) is a widely expressed member of the PPAR nuclear receptor family, which controls lipid homeostasis (1,2). In response to various ligands, PPAR proteins heterodimerize with retinoid X receptors (RXRs) in order to bind DNA and regulate target genes (3,4). PPARδ plays a role in many different biological functions, including cholesterol efflux, embryo implantation, preadipocyte proliferation, and wound healing (5-8). PPARδ has been implicated in colorectal cancer (CRC), as it is normally downregulated by APC, a tumor suppressor frequently knocked out in CRCs (9). More recently, high fat diets have been found to induce PPARδ in intestinal stem cells and progenitors, increasing their tumorigenicity (10). Furthermore, in Huntington's disease (HD) mouse models, it was shown that PPARδ was unable bind to huntingtin protein when mutated, which repressed its function. Agonist-induced activation of PPARδ in HD model mice improved cognitive function and increased survival time (11).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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