PRDM14 (E1D5S) XP® Rabbit mAb #83527
- WB
- IP
- IF
- F
- ChIP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 70 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
- F-Flow Cytometry
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:400 |
| Flow Cytometry (Fixed/Permeabilized) | 1:800 |
| Chromatin IP | 1:50 |
| Chromatin IP-seq | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
- Yamaji, M. et al. (2008) Nat Genet 40, 1016-22.
- Chia, N.Y. et al. (2010) Nature 468, 316-20.
- Tsuneyoshi, N. et al. (2008) Biochem Biophys Res Commun 367, 899-905.
- Ma, Z. et al. (2011) Nat Struct Mol Biol 18, 120-7.
- Okashita, N. et al. (2014) Development 141, 269-80.
- Yamaji, M. et al. (2013) Cell Stem Cell 12, 368-82.
- Nady, N. et al. (2015) Elife 4, e10150.
- Tu, S. et al. (2016) Nature 534, 387-90.
- Iseki, H. et al. (2016) Stem Cells 34, 322-33.
- Moelans, C.B. et al. (2010) Mod Pathol 23, 1029-39.
- Nishikawa, N. et al. (2007) Cancer Res 67, 9649-57.
- Carofino, B.L. et al. (2013) Dis Model Mech 6, 1494-506.
- Liu, B. et al. (2010) Zhongguo Fei Ai Za Zhi 13, 867-72.
- Zhang, T. et al. (2013) Med Oncol 30, 605.
- Moriya, C. et al. (2017) Carcinogenesis 38, 638-648.
Limited Uses
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