Render Target: STATIC
Render Timestamp: 2024-12-20T10:39:19.472Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:59:55.169
Product last modified at: 2024-12-02T07:00:13.979Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

PRDM16 (F3K9T) Rabbit mAb #16212

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 170
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PRDM16 (F3K9T) Rabbit mAb recognizes endogenous levels of total PRDM16 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro666 of human PRDM16 protein.

    Background

    PRDM16 is a transcription factor that is structurally characterized by a conserved N-terminal PRDI-BF1 and RIZ1 homology (PR) domain and a variable number of zinc fingers (1,2). The PR domain bears similarities to the catalytic suppressor of variegation 3–9, enhancer of zeste, and trithorax (SET) domain that is found in a group of histone methyltransferases (1,3). Indeed, PRDM16 has been shown to catalyze mono-methylation of histone 3 at lysine (H3K9me1) (4). Human PRDM16 encodes a protein with four isoforms with the most extensively studied isoforms being the full-length PRDM16 and the PR-lacking isoform that is generated by a transcript derived from an internal promoter (5). PRDM16 is involved in the regulation of various biological processes, including adipose biology and energy homeostasis. This protein is also expressed in hematopoietic stem cells (HSCs) and plays a key role in the establishment and maintenance of these cells (6,7). More recently, PRDM16 was shown to be a suppressor of lung adenocarcinoma metastasis, and its low expression predicted poor prognosis of lung adenocarcinoma patients (8).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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