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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Prdx1 Antibody #8732

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Filter:
  • WB
  • IP
Western Blotting Image 1: Prdx1 Antibody
Western blot analysis of extracts from various cell lines using Prdx1 Antibody.
This product is discontinued

Inquiry Info. # 8732

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Supporting Data

REACTIVITY H M Mk
SENSITIVITY Endogenous
MW (kDa) 21
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • Mk-Monkey 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Prdx1 Antibody recognizes endogenous levels of total Prdx1 protein. This antibody does not cross-react with Prdx2 protein.

Species Reactivity:

Human, Mouse, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro108 of human Prdx1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Prdx1 belongs to a family of non-seleno peroxidases which function as H2O2 scavengers. All 6 prdx isoforms share a conserved N-terminal cysteine (Cys51) which is oxidized by H2O2 to Cysteine-Sulfenic acid (Cys51-SOH) and, in turn, reacts with Cys172-SH of the other prdx forming a disulfide dimer that protects it from degradation (1-3). Abnormally high levels of H2O2 cause Prdx1 to form an oligomeric chaperon that loses its peroxidase activity (4). Prdxs have been reported to bind to JNK and c-Abl and regulate their kinase acitivity (5,6). Prdx1 was shown to bind to PTEN and regulate its phosphatase activity in conditions of mild or no cellular stress, hence preventing Akt-driven transformation by protecting PTEN from oxidation-induced inactivation (7).
For Research Use Only. Not For Use In Diagnostic Procedures.
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