Render Target: STATIC
Render Timestamp: 2024-11-21T12:47:55.141Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-10-15 20:01:13.007
Product last modified at: 2024-11-08T23:30:10.363Z
View Featured Offers >>
1% for the planet logo
PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a
  1. Products /
  2. Primary Antibodies /
  3. Presynaptic Vesicle Cycle Antibody Sampler Kit

Presynaptic Vesicle Cycle Antibody Sampler Kit #33738

    Product Information

    Kit Usage Information

    Protocols

    Product Description

    The Presynaptic Vesicle Cycle Antibody Sampler Kit provides an economical means of detecting expression of key synaptic vesicle proteins. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

    Specificity / Sensitivity

    Each antibody in the Presynaptic Vesicle Cycle Antibody Sampler Kit detects endogenous levels of its target protein. VAMP2 (D6O1A) Rabbit mAb does not cross-react with VAMP1 protein and recognizes VAMP2 protein by IF-IC analysis in mouse and rat cells. Complexin-1/2 (D8A6E) Rabbit mAb recognizes endogenous levels of total complexin-1 and complexin-2 protein. Complexin-1 (D5Q5H) Rabbit mAb does not cross-react with complexin-2. Synaptotagmin-1 (D33B7) Rabbit mAb may also cross-react with an unidentified protein of approximately 45 kDa. The antigen for Synapsin-1 (D12G5) XP® Rabbit mAb is 100% conserved between human synapsin-1a and synapsin-1b.

    Source / Purification

    Complexin-1 (D5Q5H) Rabbit mAb is produced by immunizing recombinant protein specific to human complexin-1 protein. Complexin-1/2 (D8A6E) Rabbit mAb is produced by immunizing rabbits with a synthetic peptide corresponding to residues surrounding Pro125 of human complexin-2 protein. VAMP2 (D6O1A) Rabbit mAb is produced by immunizing recombinant protein specific to the amino terminus of human VAMP2 protein. All other monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to Asp110 of human SNAP25, Val237 of human syntaxin 1A, Gly299 of human synaptophysin, Arg400 of human synaptotagmin-1, Gln483 of human synapsin-1, and Tyr157 of human Munc18-1.

    Background

    The synapse is the unit of information transfer in the brain between neurons (1). Synaptic transfer is initiated by action potential-initiated synaptic vesicles fusion at the presynaptic terminal to release neurotransmitters. The basic molecular machinery that drives all membrane fusion events in all cells include N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins that form a trans-ternary complex between to-be-fused membranes. The zippering up of SNARE proteins provides the energy required for membrane fusion. At the synapse, the SNARE proteins include 25 kDa synaptosome-associated protein (SNAP25), syntaxin 1A (STX1A), and vesicle-associated membrane protein 2 (VAMP2, also called synaptobrevin). Neurotransmitter release, however, is a highly regulated process that occurs in response to action potential-initiated Ca2+-influx. Regulation of synaptic vesicle fusion is mediated by SNARE-binding proteins like complexin and the Ca2+-associated vesicle protein, synaptotagmin-1, that cooperatively function to initiate Ca2+-induced synaptic vesicle fusion. The synaptic vesicle cycle represents a cycle of events that prime the synaptic vesicle before fusion and retrieve fused membrane after full fusion. Synaptic vesicle-associated proteins like synaptophysin and synapsin-1, as well as target membrane proteins like Munc18-1, play a complex role in priming synaptic vesicles to be fusion competent.   

    Database Links

    UniProt ID: P60880 , P21579 , P08247 , Q6PUV4 , P63027 , Q16623 , O14810 , P61764 , P17600


    Entrez-Gene Id: 6616 , 6857 , 6855 , 10814 , 6844 , 6804 , 10815 , 6812 , 6853


    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    XP is a registered trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.