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R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Prostate Specific Membrane Antigen (D4S1F) Rabbit mAb #12702

Filter:
  • WB
  • IF
Western Blotting Image 1: Prostate Specific Membrane Antigen (D4S1F) Rabbit mAb
Western blot analysis of extracts from various cell lines using Prostate Specific Membrane Antigen (D4S1F) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower)

To Purchase # 12702

Cat. # Size Qty. Price
12702T 20 µl
$153
12702S 100 µl
$339

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 100
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunofluorescence (Immunocytochemistry) 1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #66648.

Protocol

Specificity / Sensitivity

Prostate Specific Membrane Antigen (D4S1F) Rabbit mAb recognizes endogenous levels of total prostate specific membrane antigen. An SDS resistant dimer at approximately 200 kDa can be detected depending on sample preparation conditions.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues in the extracellular domain of human prostate specific membrane antigen protein.

Background

Prostate specific membrane antigen (PSMA, also known as FOLH1), a type II transmembrane protein of the M28 family, has both folate hydrolase and N-acetylated-alpha-linked acidic dipeptidase activity. PSMA was originally identified in the LNCaP cell line, which was derived from a prostate adenocarcinoma lymph node metastasis (1,2). PSMA is an established prostate cancer marker (3); however, it is expressed in other tissues, including kidney, liver, and urinary bladder (4), and it is associated with tumor neovasculature (5) as well. Research studies suggest that PSMA is both a potential diagnostic readout and therapeutic target (6-8).
For Research Use Only. Not For Use In Diagnostic Procedures.
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