PROX1 (D2J6J) Rabbit mAb (BSA and Azide Free) #45546

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Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	85
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IHC-Immunohistochemistry
IF-Immunofluorescence

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

This product is the carrier free version of product #14963. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

Formulation

Supplied in 1X PBS (10 mM Na₂HPO₄, 3 mM KCl, 2 mM KH₂PO₄, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

For standard formulation of this product see product #14963.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

PROX1 (D2J6J) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total PROX1 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu567 of human PROX1 protein.

Background

Prospero homeobox protein 1 (PROX1) is a transcription factor known for its roles in organ development and lymphangiogenesis. It plays a critical role in the development of the CNS, lens, retina, liver, pancreas, and heart, and is considered to be the master regulator of the lymphatic system (1,2). PROX1 initiates the differentiation of lymphatic vasculature from the cardinal vein, where it is regulated by Sox18 (3,4). The PROX1 suppressor COUP-TFII represses the Notch pathway in venous endothelium, which prevents arterialization (4). HIF-1α and HIF-1β mediated hypoxia induces PROX1, which suggests a means of promoting lymphangiogenesis. Since the tumor microenvironment is typically hypoxic, regulation of PROX1 by hypoxia may also explain the up-regulation of this transcription factor in some cancers (2). PROX1 promotes colon cancer progression by down-regulating E-cadherin via miR-9, which promotes epithelial-mesenchymal transition (EMT) and metastasis (5). The PROX1 protein can act as a tumor suppressor in cases of hepatocellular carcinoma. PROX1 represses transcription of TWIST1, a transcription factor that promotes metastasis by binding the E-cadherin promoter. The function of PROX1 in other cancers is an area of active research (6).

Database Links

UniProt ID: Q92786

Entrez-Gene Id: 5629

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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