PSMC3/TBP1 Antibody #13923
- WB
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 46, 48 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
The base of the eukaryotic proteasome 19S/PA700 RP contains six AAA-ATPase subunits (PSMC1-PSMC6) that bind directly to the 20S CP α-ring. These 19S RP ATPases are thought to assemble into a heterohexameric, pore-like structure that forms part of the substrate translocation channel. Energy derived from ATP hydrolysis by the AAA-ATPases is utilized for substrate unfolding and translocation, which is required for degradation of ubiquitinated folded proteins within the central chamber of the 20S CP formed by β-subunits (3-5). The human immunodeficiency virus Tat-Binding Protein 1 (PSMC3, TBP1) is a 19S AAA-ATPase subunit that functions as a transcriptional activator (6-8). Research studies demonstrate that PSMC3/TBP1 may act as a tumor suppressor by promoting pVHL-dependent degradation of HIF1α (9) and protecting p14-ARF from proteasomal degradation (10).
- Finley, D. (2009) Annu Rev Biochem 78, 477-513.
- Lee, M.J. et al. (2011) Mol Cell Proteomics 10, R110.003871.
- Groll, M. et al. (2000) Nat Struct Biol 7, 1062-7.
- Braun, B.C. et al. (1999) Nat Cell Biol 1, 221-6.
- Liu, C.W. et al. (2002) J Biol Chem 277, 26815-20.
- Nelbock, P. et al. (1990) Science 248, 1650-3.
- Ohana, B. et al. (1993) Proc Natl Acad Sci U S A 90, 138-42.
- Satoh, T. et al. (2009) Endocrinology 150, 3283-90.
- Corn, P.G. et al. (2003) Nat Genet 35, 229-37.
- Pollice, A. et al. (2007) Oncogene 26, 5154-62.
Limited Uses
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