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Render Timestamp: 2025-01-21T03:40:46.961Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-08-30 10:36:25.204
Product last modified at: 2025-01-01T09:04:43.759Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

PVR/CD155 (D8A5G) Rabbit mAb (BSA and Azide Free) #31235

Filter:
  • WB
  • IHC
Western Blotting Image 1: PVR/CD155 (D8A5G) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from various cell lines using PVR/CD155 (D8A5G) Rabbit mAb (upper) and β-actin (D6A8) Rabbit mAb #8457 (lower). Data were generated using the standard formulation of this product.

To Purchase # 31235

Cat. #

Size

31235SF
100 µg

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 60-80
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
Species Cross-Reactivity Key:
  • H-Human 

Product Information

Product Usage Information

This product is the carrier free version of product #81254. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

Formulation

Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

For standard formulation of this product see product #81254.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

PVR/CD155 (D8A5G) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total CD155 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn188 of human CD155 protein.

Background

Poliovirus receptor (PVR), also known as CD155 and nectin-like 5 (Necl-5), is an immunoglobulin-like, transmembrane glycoprotein originally described as a mediator of poliovirus attachment to cells and later identified as important in adherens junction formation. PVR binds nectin-3 and interacts with integrin αvβ3 and PDGFR to regulate integrin clustering and focal contact formation at the leading edge of migrating cells (1,2). Research studies demonstrate that PVR and nectin-3 regulate contact inhibition during cell motility and proliferation in transformed 3T3 cells (3). Additional research indicates that PVR expression may play a role in the invasiveness of lung adenocarcinoma (4,5). In the immune system, CD155 plays a role in natural killer (NK) cell-mediated cytotoxicity (6).
For Research Use Only. Not For Use In Diagnostic Procedures.
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