Render Target: STATIC
Render Timestamp: 2024-11-12T10:21:09.524Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-09-30 01:59:03.286
Product last modified at: 2024-09-30T08:02:00.624Z
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

PXR (E4S1W) Rabbit mAb #44646

Filter:
  • WB
  • IP
  • ChIP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 45
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • ChIP-Chromatin Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 × 10^6 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Chromatin IP 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PXR (E4S1W) Rabbit mAb recognizes endogenous levels of total PXR protein. This antibody detects a 70 kDa band of unknown origin.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant human PXR protein.

    Background

    The pregnane X receptor (PXR) is part of the nuclear hormone receptor superfamily and is a master regulator of xenobiotic metabolism (1). PXR was initially described as being activated by pregnanes and inducing expression of the CYP3A family of steroid hydroxylases (2,3). Similar to PPAR and CAR nuclear receptors, PXR utilizes RXR as a heterodimeric partner and controls target hepatic enzyme genes to control liver metabolism in response to a wide variety of drugs (4). However, PXR is strikingly evolutionarily divergent, and the ligand-binding domain only shares around 75% identity across species. Compounds that readily activate human PXR, such as rifampicin, have little activity in rodents (5,6). In humans, in addition to naturally occurring steroids, PXR is activated by numerous xenobiotics, including anti-cancer drugs paclitaxel and tamoxifen, the HIV protease inhibitor ritonavir, and the anti-diabetic troglitazone (5,7-9). PXR has been implicated in numerous conditions, including inflammatory bowel disease, atherosclerosis, and non-alcoholic fatty liver disease (NAFLD) (10-12). PXR plays a protective role in many tumor types and its expression is generally correlated with better prognosis (13-15).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.