Render Target: STATIC
Render Timestamp: 2024-12-03T12:07:58.858Z
Commit: cd2fae6ca3f811b1ddb1df24ac291ed56d5d501b
XML generation date: 2024-11-20 22:01:11.788
Product last modified at: 2024-11-26T13:15:14.932Z
View Featured Offers >>
1% for the planet logo
PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a
  1. Products /
  2. Primary Antibodies /
  3. Pyroptosis Antibody Sampler Kit

Pyroptosis Antibody Sampler Kit #43811

    Product Information

    Kit Usage Information

    Protocols

    Product Description

    The Pyroptosis Antibody Sampler Kit provides an economical means of detecting proteins that are used as readouts for pyroptosis. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

    Specificity / Sensitivity

    Gasdermin D (E8G3F) Rabbit mAb recognizes endogenous levels of total Gasdermin D protein. This antibody recognizes the 30 kDa amino terminal fragment produced during pyroptosis by caspase-1, a 43 kDa fragment produced by caspase-3, as well as a 21 kDa fragment produced by cleavage at both sites. Cleaved Gasdermin D (Asp275) (E7H9G) Rabbit mAb detects the N-terminal fragment of human Gasdermin D protein only when cleaved at Asp275. Caspase-1 (D7F10) Rabbit mAb detects endogenous levels of full length human caspase-1 and the activated p20 subunit by overexpression. Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb detects the p20 subunit of human caspase-1 upon cleavage at Asp275. IL-1β (D3U3E) Rabbit mAb can recognize endogenous levels of full-length protein, but does not detect endogenous levels of the mature IL-1β. It can detect up to 100 pg of recombinant mature IL-1β. Cleaved IL-1β (Asp116) (D3A3Z) Rabbit mAb detects endogenous levels of mature IL-1β protein when cleaved at Asp116. Caspase-4 Antibody detects endogenous levels of total caspase-4 and intermediate forms at 40 and 32 kDa. Caspase-5 (D3G4W) Rabbit mAb detects endogenous levels of caspase-5. HMGB1 (D3E5) Rabbit mAb detects endogenous levels of total HMGB1. It does not cross-react with other HMGB proteins, including HMGB2 and HMGB3.

    Source / Purification

    Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Trp130 of human Gasdermin D, residues within the p20 subunit of caspase-1, Asp297 of human caspase-1, Asp116 of human IL-1β, Pro154 of human caspase-5, and Ala137 of human HMGB1. IL-1β (D3U3E) Rabbit mAb is produced by immunizing animals with a recombinant IL-1β protein. Polyclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile125 of human caspase-4. Polyclonal antibodies are purified by Protein A and peptide affinity chromatography.

    Background

    Pyroptosis is a regulated pathway of cell death with morphological features of necrosis, including cell swelling, plasma membrane pore formation, and engagement of an inflammatory response with the release of a number of damage-associated molecular patterns (DAMPs), such as HMGB1 and inflammatory cytokines like IL-1β and IL-18 (1,2). Pyroptosis is generally induced in cells of the innate immune system, such as monocytes, macrophages, and dendritic cells in the presence of pathogen-associated molecular patterns (PAMPs) expressed on microbial pathogens or by cell-derived DAMPs. It is induced through assembly of inflammasomes triggering proteolytic activation of caspase-1 which then cleaves inflammatory cytokines like IL-1β and IL-18 to their mature forms (3). A critical feature of pyroptosis is the cleavage of Gasdermin D by caspase-1 and mouse caspase-11 (or human caspase-4/5) (4-6). Upon cleavage, the N-terminal fragment of Gasdermin D oligomerizes to form a pore, allowing secretion of inflammatory DAMPs and cytokines. Canonical inflammasome assembly typically consists of a cytosolic-pattern recognition receptor (PPR; a nucleotide binding domain and leucine-rich repeat [NLR] or AIM2-like family members), an adaptor protein (ASC/TMS1), and pro-caspase-1. Distinct inflammasome complexes can recognize distinct PAMPs and DAMPs to trigger pyroptosis. The best characterized pathway triggered by the NLR, NLRP3, occurs through a two-step process. The first step is a priming signal, NF-κB is activated to induce the expression of a number of inflammasome components including NLRP3, pro-IL-1β, and pro-IL-18. In the second activation step, caspase-1 is activated and Gasdermin D and cytokines are proteolytically activated. In a non-canonical pathway, caspase-4 and caspase-5 can directly trigger Gasdermin D cleavage in monocytes following LPS stimulation (5,7).

    Database Links

    UniProt ID: P01584 , P29466 , P49662 , P57764 , P51878 , P09429


    Entrez-Gene Id: 3553 , 834 , 837 , 79792 , 838 , 3146


    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.